# Sequence Coverage Plotting¶

The scov subcommand of the conkit-plot script is used to plot the coverage plot of the multiple sequence alignment.

\$> conkit-plot scov toxd/toxd.a3m a3m


The following plot will be produced. Your alignment coverage is shown with the black line with each point corresponding to a residue in the alignment. The other two lines give you indicators of how good your alignment is.

If parts or all of your coverage fall below the “5 x Nresidues” line, the suitability for covariance-based contact prediction is very low. If most residues in the alignment are well above the “5 x Nresidues” line, possibly even the “20 x Nresidues”, and bigger chunks are below, then you might want to consider re-defining your sequence boundaries to predict contacts only for the well-covered area.

If most residues in your alignment have a better coverage than the “20 x Nresidues” line, the alignment is well-suited for covariance-based predictions.